Grotz V L, Ji S, Kline S A, Goldstein B D, Witz G
Joint Graduate Program in Toxicology, Rutgers University/UMDNJ-Robert Wood Johnson Medical School, Piscataway.
Toxicol Lett. 1994 Feb 15;70(3):281-90. doi: 10.1016/0378-4274(94)90122-8.
Perfusate from rat livers perfused with benzene (approximately 0.7-7 x 10(-4) M) or trans,trans-muconaldehyde (MUC) (10(-4) M) was extracted and analyzed by reverse-phase HPLC. Based on retention time and co-elution experiments, benzene was found to be metabolized to trans,trans-muconic acid, a urinary ring-opened metabolite of benzene and a major in vivo and in vitro metabolite of MUC. These data demonstrate that benzene ring-opening occurs in the liver. Following perfusion with MUC (a microsomal hematotoxic metabolite of benzene), trans,trans-muconic acid and three other MUC metabolites were detected in the perfusate extract, suggesting that these metabolites would be present in the circulation following metabolism of MUC.
用苯（约0.7 - 7×10⁻⁴ M）或反，反-粘康醛（MUC）（10⁻⁴ M）灌注大鼠肝脏后，收集灌注液，并用反相高效液相色谱法进行提取和分析。基于保留时间和共洗脱实验，发现苯被代谢为反，反-粘康酸，这是苯的一种尿中环开环代谢物，也是MUC在体内和体外的主要代谢物。这些数据表明肝脏中发生了苯的开环反应。在用MUC（苯的一种微粒体血液毒性代谢物）灌注后，在灌注液提取物中检测到了反，反-粘康酸和其他三种MUC代谢物，这表明这些代谢物在MUC代谢后会出现在循环中。
