Ibebunjo C
Department of Clinical Veterinary Medicine, University of Cambridge, UK.
Vet Res Commun. 1993;17(3):171-82. doi: 10.1007/BF01839162.
This study was designed to determine the histochemical properties, size and composition of fibres in the diaphragm, intercostal and abdominal muscles of goats to clarify whether reported similarities in respiratory muscle physiology between goats and humans have a structural basis. Serial sections (10 microns) of muscular tissue from adult female goats were stained for myosin adenosine triphosphatase and reduced nicotinamide adenine dinucleotide dehydrogenase-tetrazolium reductase activities; the fibres were classified into type I, IIA and IIB; and their mean diameter and composition were determined. Abdominal and intercostal muscles contained types I, IIA and IIB fibres in the ratio 1:1:1, and the mean diameter of the fibres ranged from 49.2 to 62.2 microns. In contrast, the diaphragm contained 58.9% type I and 41.1% type II fibres, and the latter could not be differentiated into types IIA and IIB. Diaphragmatic fibres were also smaller (36.9-40.9 microns). These findings contrast with those in humans, where the diaphragm, intercostal and abdominal muscles contain > 50% type I fibres and have fibres of identical diameter. The differences in fibre characteristics between the diaphragm, intercostal and abdominal muscles of goats and the differences between goats and humans need to be taken into consideration in interpreting the results from studies in respiratory muscle physiology.
本研究旨在确定山羊膈肌、肋间肌和腹肌中纤维的组织化学特性、大小和组成，以阐明所报道的山羊与人类呼吸肌生理学上的相似性是否具有结构基础。对成年雌性山羊肌肉组织的连续切片（10微米）进行肌球蛋白三磷酸腺苷酶和还原型烟酰胺腺嘌呤二核苷酸脱氢酶 - 四氮唑还原酶活性染色；将纤维分为I型、IIA型和IIB型；并测定其平均直径和组成。腹肌和肋间肌中I型、IIA型和IIB型纤维的比例为1:1:1，纤维的平均直径在49.2至62.2微米之间。相比之下，膈肌含有58.9%的I型纤维和41.1%的II型纤维，且后者无法区分为IIA型和IIB型。膈肌纤维也较小（36.9 - 40.9微米）。这些发现与人类的情况形成对比，人类的膈肌、肋间肌和腹肌含有>50%的I型纤维且纤维直径相同。在解释呼吸肌生理学研究结果时，需要考虑山羊膈肌、肋间肌和腹肌之间纤维特征的差异以及山羊与人类之间的差异。
