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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Fitzpatrick P J, Sheehan D

Department of Biochemistry, University of College Cork, Mardyke, Ireland.

Xenobiotica. 1993 Aug;23(8):851-61. doi: 10.3109/00498259309059413.

Glutathione S-transferase isoenzymes from Mytilus edulis and M. galloprovincialis have been partially purified by glutathione-sepharose affinity chromatography followed by Mono Q anion exchange fast protein liquid chromatography (f.p.l.c.). 2. The tissue distribution of glutathione S-transferase in M. edulis has been studied. Using 1-chloro-2,4-dinitrobenzene as substrate, highest specific activity is observed in the gill, the main feeding organ. Affinity-purified extracts of this organ give a characteristic f.p.l.c. profile. A similar profile is obtained with affinity-purified extracts of the digestive gland of M. galloprovincialis. 3. The subunit structure of the purified isoenzymes has been studied by SDS polyacrylamide gel electrophoresis and reversed-phase h.p.l.c. The subunits have similar molecular weights and h.p.l.c. retention times to rat glutathione S-transferases.

紫贻贝和地中海贻贝中的谷胱甘肽S-转移酶同工酶已通过谷胱甘肽琼脂糖亲和层析，随后进行Mono Q阴离子交换快速蛋白质液相色谱（f.p.l.c.）进行了部分纯化。2. 研究了紫贻贝中谷胱甘肽S-转移酶的组织分布。以1-氯-2,4-二硝基苯为底物，在主要摄食器官鳃中观察到最高的比活性。该器官的亲和纯化提取物给出了特征性的f.p.l.c.图谱。用亲和纯化的地中海贻贝消化腺提取物也得到了类似的图谱。3. 通过SDS聚丙烯酰胺凝胶电泳和反相h.p.l.c.研究了纯化的同工酶的亚基结构。这些亚基的分子量和h.p.l.c.保留时间与大鼠谷胱甘肽S-转移酶相似。

Fitzpatrick P J, Sheehan D

Department of Biochemistry, University of College Cork, Mardyke, Ireland.

Xenobiotica. 1993 Aug;23(8):851-61. doi: 10.3109/00498259309059413.

Glutathione S-transferase isoenzymes from Mytilus edulis and M. galloprovincialis have been partially purified by glutathione-sepharose affinity chromatography followed by Mono Q anion exchange fast protein liquid chromatography (f.p.l.c.). 2. The tissue distribution of glutathione S-transferase in M. edulis has been studied. Using 1-chloro-2,4-dinitrobenzene as substrate, highest specific activity is observed in the gill, the main feeding organ. Affinity-purified extracts of this organ give a characteristic f.p.l.c. profile. A similar profile is obtained with affinity-purified extracts of the digestive gland of M. galloprovincialis. 3. The subunit structure of the purified isoenzymes has been studied by SDS polyacrylamide gel electrophoresis and reversed-phase h.p.l.c. The subunits have similar molecular weights and h.p.l.c. retention times to rat glutathione S-transferases.

紫贻贝和地中海贻贝中的谷胱甘肽S-转移酶同工酶已通过谷胱甘肽琼脂糖亲和层析，随后进行Mono Q阴离子交换快速蛋白质液相色谱（f.p.l.c.）进行了部分纯化。2. 研究了紫贻贝中谷胱甘肽S-转移酶的组织分布。以1-氯-2,4-二硝基苯为底物，在主要摄食器官鳃中观察到最高的比活性。该器官的亲和纯化提取物给出了特征性的f.p.l.c.图谱。用亲和纯化的地中海贻贝消化腺提取物也得到了类似的图谱。3. 通过SDS聚丙烯酰胺凝胶电泳和反相h.p.l.c.研究了纯化的同工酶的亚基结构。这些亚基的分子量和h.p.l.c.保留时间与大鼠谷胱甘肽S-转移酶相似。