de Herdt P, Haesebrouck F, Devriese L A, Ducatelle R
Laboratory of Veterinary Bacteriology & Mycology, Faculty of Veterinary Medicine, University of Gent, Belgium.
Zentralbl Veterinarmed B. 1993 Sep;40(7):494-500. doi: 10.1111/j.1439-0450.1993.tb00168.x.
Indirect ELISA techniques for the detection of antibodies to S. bovis serotypes 1, 2 and 3 in pigeon plasma were developed. Whole formaldehyde-inactivated bacteria were used as coating antigens. Bound antibodies were detected with peroxidase-conjugated rabbit anti-pigeon serum and OPD and H2O2 as chromogen and substrate, respectively. ELISA was used to determine the prevalence of antibodies to S. bovis serotype 1 in healthy Belgian racing pigeons. Antibodies were demonstrated in 83 (= 37%) of 225 samples tested. Results indicate that pigeons may build up plasma antibodies in the absence of clinical signs resembling septicaemia.
开发了用于检测鸽血浆中牛链球菌1、2和3型抗体的间接ELISA技术。使用完全甲醛灭活的细菌作为包被抗原。分别用辣根过氧化物酶标记的兔抗鸽血清以及OPD和H₂O₂作为显色剂和底物来检测结合的抗体。采用ELISA法测定健康比利时赛鸽中牛链球菌1型抗体的流行情况。在检测的225份样本中，有83份（= 37%）显示有抗体。结果表明，鸽子在没有类似败血症临床症状的情况下可能会产生血浆抗体。
