由次氯酸钠和氯化锰引发的钙离子激活光蛋白水母发光蛋白的发光现象。
Luminescence of Ca(2+)-activated photoprotein obelin initiated by NaOCl and MnCl2.
作者信息
Vysotski E S, Trofimov K P, Bondar' V S, Gitelson J I
机构信息
Laboratory of Photobiology, Russian Academy of Sciences, Krasnoyarsk.
出版信息
J Biolumin Chemilumin. 1993 Nov-Dec;8(6):301-5. doi: 10.1002/bio.1170080603.
The luminescence of obelin is initiated by NaOCl in a reaction mixture containing no calcium. The addition of Mn2+ enhances the light emission > 300-fold. Sodium azide and histidine, as singlet oxygen quenchers, inhibit NaOCl-activated obelin luminescence in the presence or absence of Mn2+. This suggests that the addition of NaOCl to the mixture causes singlet oxygen formation (stimulated by Mn2+ ions), and singlet oxygen initiates the light-emitting reaction.
在不含钙的反应混合物中，次氯酸钠可引发水母发光蛋白的发光。添加锰离子可使发光增强300倍以上。叠氮化钠和组氨酸作为单线态氧猝灭剂，无论有无锰离子存在，均能抑制次氯酸钠激活的水母发光蛋白发光。这表明向混合物中添加次氯酸钠会导致单线态氧的形成（由锰离子刺激），且单线态氧引发发光反应。
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