Ogino K, Koda F, Miyata K
Eye Clinic, Showa General Hospital, Kodaira, Japan.
Nippon Ganka Gakkai Zasshi. 1993 Nov;97(11):1286-91.
An experimental study was performed to estimate the degree of corneal endothelial damage caused by ultrasound during phacoemulsification. Cultured corneal endothelium prepared from bovine eyes was exposed to ultrasound. Dead cell rates were determined by counting the number of cells positively stained by trypan blue under various conditions of ultrasonic radiation. Dead cell rate increased from 13 +/- 2% to 39 +/- 4% (n = 5, Mean +/- S.D.) as ultrasonic duration was prolonged from 15 seconds to 45 seconds. Dead cell rate increased from 6 +/- 1% to 39 +/- 4% as ultrasonic power increased from 60% to 100%. Dead cell rate increased from 8 +/- 1% to 44 +/- 11% as the distance of the phaco tip decreased from 7 mm to 4 mm. Dead cell rate increased from 4 +/- 2% to 35 +/- 7% as the angle of the phaco tip increased from 30 degrees to 90 degrees. We confirmed that endothelial damage depended on ultrasonic duration, ultrasonic power, the distance of the phaco tip, and the angle of the phaco tip. We suggest that the endothelium is damaged mainly by shock waves emanating from the phaco tip.
进行了一项实验研究，以评估超声乳化过程中超声对角膜内皮造成的损伤程度。将从牛眼制备的培养角膜内皮暴露于超声下。在不同超声辐射条件下，通过计数经台盼蓝阳性染色的细胞数量来确定死细胞率。随着超声持续时间从15秒延长至45秒，死细胞率从13±2%增至39±4%（n = 5，平均值±标准差）。随着超声功率从60%增至100%，死细胞率从6±1%增至39±4%。随着超声乳化头距离从7毫米降至4毫米，死细胞率从8±1%增至44±11%。随着超声乳化头角度从30度增至90度，死细胞率从4±2%增至35±7%。我们证实，内皮损伤取决于超声持续时间、超声功率、超声乳化头距离以及超声乳化头角度。我们认为，内皮损伤主要是由超声乳化头发出的冲击波所致。
