Rodtong S, Dobbinson S, Thode-Andersen S, McConnell M A, Tannock G W
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Appl Environ Microbiol. 1993 Nov;59(11):3871-7. doi: 10.1128/aem.59.11.3871-3877.1993.
Four DNA probes were derived that hybridized specifically to DNA from Lactobacillus acidophilus O. The probes were constructed by randomly cloning lactobacillus DNA in plasmid vector pBR322. Two of the probes (pSR1 and pSR2) were composed of vector and plasmid DNA inserts (3.6 and 1.6 kb, respectively); the others (pSR3 and pSR4) were composed of vector and chromosomally derived inserts (6.9 and 1.4 kb, respectively). The probes were used to enumerate, by colony hybridization, strain O in digestive tract samples collected from piglets inoculated 24 hours previously with a culture of the strain. The probes did not hybridize to DNA from lactobacilli inhabiting the digestive tract of uninoculated piglets. Strain O made up about 10% of the total lactobacillus population of the pars esophagea and about 20% of the population in other digestive tract samples.
获得了四种DNA探针，它们能与嗜酸乳杆菌O的DNA特异性杂交。这些探针是通过将乳杆菌DNA随机克隆到质粒载体pBR322中构建而成的。其中两种探针（pSR1和pSR2）由载体和质粒DNA插入片段组成（分别为3.6 kb和1.6 kb）；另外两种（pSR3和pSR4）由载体和染色体来源的插入片段组成（分别为6.9 kb和1.4 kb）。通过菌落杂交，利用这些探针来计数从24小时前接种该菌株培养物的仔猪采集的消化道样本中的O菌株。这些探针未与未接种仔猪消化道中栖息的乳杆菌的DNA杂交。O菌株在食管部分的总乳杆菌菌群中约占10%，在其他消化道样本的菌群中约占20%。
