单核细胞增生李斯特菌特异性核苷酸序列的分离与鉴定。
Isolation and characterization of Listeria monocytogenes-specific nucleotide sequences.
作者信息
Chen J, Brosch R, Luchansky J B
机构信息
Department of Food Microbiology and Toxicology, Food Research Institute, Madison, Wisconsin.
出版信息
Appl Environ Microbiol. 1993 Dec;59(12):4367-70. doi: 10.1128/aem.59.12.4367-4370.1993.
Abstract
Subtracter probe hybridization was used to screen a partial genomic library of a clinical isolate of Listeria monocytogenes. Three clones that hybridized with genomic DNA from 174 strains of L. monocytogenes but not with genomic DNA from 32 strains representing other Listeria spp. were recovered. These data establish the utility of subtracter probe hybridization for recovering L. monocytogenes-specific sequences.
摘要
减法探针杂交技术被用于筛选单核细胞增生李斯特菌临床分离株的部分基因组文库。回收了三个与174株单核细胞增生李斯特菌的基因组DNA杂交，但不与代表其他李斯特菌属的32株菌株的基因组DNA杂交的克隆。这些数据证实了减法探针杂交技术在回收单核细胞增生李斯特菌特异性序列方面的实用性。
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