Nalbantoglu B, Hirasawa M, Moomaw C, Nguyen H, Knaff D B, Allen R
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061.
Biochim Biophys Acta. 1994 Jan 4;1183(3):557-61. doi: 10.1016/0005-2728(94)90086-8.
The nucleotide sequences of two cDNA clones, totalling 4948 bp in length, encoding 98% of the 1483 amino acids of the mature form of the ferredoxin-dependent glutamate synthase of spinach chloroplasts have been determined. The amino-terminal sequence of the enzyme has been determined by direct sequencing of the protein. The deduced amino-acid sequence of the spinach enzyme is 83% identical to that of the ferredoxin-dependent maize enzyme and shows significant sequence homology to two prokaryotic NAD(P)H-dependent glutamate synthases. Analysis of spinach genomic DNA indicates the presence of a single-copy gene for the spinach enzyme. Northern analysis reveals the presence of a single 5.5 kb transcript, which is present in higher levels in young spinach leaves than in older leaves.
已测定了两个cDNA克隆的核苷酸序列，其长度总计为4948 bp，编码菠菜叶绿体铁氧还蛋白依赖性谷氨酸合酶成熟形式1483个氨基酸中的98%。该酶的氨基末端序列已通过蛋白质直接测序确定。菠菜酶推导的氨基酸序列与铁氧还蛋白依赖性玉米酶的序列有83%的同一性，并且与两种原核NAD(P)H依赖性谷氨酸合酶有显著的序列同源性。对菠菜基因组DNA的分析表明存在该菠菜酶的单拷贝基因。Northern分析揭示存在一个单一的5.5 kb转录本，其在幼嫩菠菜叶中的含量高于老叶。
