Miyake K, Onaka H, Horinouchi S, Beppu T
Department of Agricultural Chemistry, University of Tokyo, Japan.
Biochim Biophys Acta. 1994 Jan 18;1217(1):97-100.
The nusG genes of Streptomyces griseus and Streptomyces coelicolor A3(2) were cloned by the DNA-probing method with synthetic oligonucleotides designed on the basis of the nucleotide sequence of the nusG gene of Streptomyces virginiae. The amino acid sequences of the NusG proteins deduced from the nucleotide sequences showed significant homologies to those from a variety of microorganisms. Nucleotide sequence analysis of the region upstream of the nusG gene of S. griseus revealed the presence of the secE gene, suggesting that secE and nusG are organized as an operon as is found in other microorganisms.
利用根据弗吉尼亚链霉菌nusG基因的核苷酸序列设计的合成寡核苷酸，通过DNA探针法克隆了灰色链霉菌和天蓝色链霉菌A3(2)的nusG基因。从核苷酸序列推导的NusG蛋白的氨基酸序列与多种微生物的氨基酸序列具有显著同源性。灰色链霉菌nusG基因上游区域的核苷酸序列分析表明存在secE基因，这表明secE和nusG如在其他微生物中那样被组织成一个操纵子。
