Navas-Castillo J, Laigret F, Hocquellet A, Chang C J, Bove J M
Laboratoire de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique et Université de Bordeaux II, Villenave d'Ornon, France.
Biochimie. 1993;75(8):675-9. doi: 10.1016/0300-9084(93)90098-d.
In order to confirm the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose in the presence of PEP. We also cloned and sequenced a DNA fragment coding for a putative polypeptide showing significant similarity with the enzyme II of the beta-glucoside PTS of Escherichia coli. Taken together, these results show that A florum possesses a fructose-PTS, but not a glucose-PTS, and that the amino acid sequence deduced from the DNA fragment is related to beta-glucoside and sucrose enzymes II of PTS from various bacteria.
为了证实柔膜菌属的弗氏无胆甾原体中存在磷酸烯醇式丙酮酸（PEP）依赖性糖磷酸转移酶系统（PTS），我们研究了该生物体的无细胞提取物在PEP存在下磷酸化葡萄糖和/或果糖的能力。我们还克隆并测序了一个DNA片段，该片段编码一种推定的多肽，与大肠杆菌β-葡萄糖苷PTS的酶II具有显著相似性。综合这些结果表明，弗氏无胆甾原体拥有果糖-PTS，但不拥有葡萄糖-PTS，并且从该DNA片段推导的氨基酸序列与来自各种细菌的PTS的β-葡萄糖苷酶和蔗糖酶II相关。
