Paoletti J, Mougel M, Tounekti N, Girard P M, Ehresmann C, Ehresmann B
Unité de Biochimie, URA 147 CNRS and U140 INSERM, Institut Gustave-Roussy, Villejuif, France.
Biochimie. 1993;75(8):681-6. doi: 10.1016/0300-9084(93)90099-e.
The genome of the Moloney murine leukemia virus (MoMuLV) is composed of two identical RNA molecules joined at their 5' ends by the dimer linkage structure (DLS). Dimerization sequences are located within the PSI encapsidation domain. We present here an overview of the work we have performed on spontaneous dimerization of a MoMuLV RNA fragment encompassing the PSI domain in order to understand the mechanism by which retroviral RNA dimerization takes place. We present kinetical, thermodynamical and conformational evidence which leads to the conclusion that the PSI domain is a structurally independent domain and that conformational changes are triggered by the dimerization process. We conclude that at least one particular region (nucleotides 278-309) of the RNA is directly involved in the process while the conformation of some other regions is changed probably because of a long-range effect.
莫洛尼鼠白血病病毒（MoMuLV）的基因组由两个相同的RNA分子组成，它们在5'端通过二聚体连接结构（DLS）相连。二聚化序列位于PSI包装结构域内。我们在此概述了我们对包含PSI结构域的MoMuLV RNA片段自发二聚化所做的工作，以便了解逆转录病毒RNA二聚化发生的机制。我们提供了动力学、热力学和构象方面的证据，这些证据得出的结论是，PSI结构域是一个结构独立的结构域，并且构象变化是由二聚化过程触发的。我们得出结论，RNA的至少一个特定区域（核苷酸278 - 309）直接参与该过程，而其他一些区域的构象变化可能是由于长程效应。
