Honjo K, Hirota Y
Laboratory of Immune Cytology, National Institute of Animal Health, Ibaraki, Japan.
J Vet Med Sci. 1993 Oct;55(5):895-7. doi: 10.1292/jvms.55.895.
The localization of lymphocytes in the lymphoid organs of cyclophosphamide (Cy) treated chickens and untreated control chickens was compared immunohistochemically using a variety of monoclonal antibodies (CT3, 2-6, 11-39, TCR1, TCR2, TCR3, L22, 11G, 3E8, B-4D-4, A-13). In the Harderian glands of Cy treated chickens, an an increase of T cells was observed, though T cells were a few in untreated controls. These increased T cells consisted of CD4 positive or CD8 positive cells. Further, these T cells were stained with TCR2 or TCR3 antibody, and a small number of cells were stained with TCR1 antibody. In other lymphoid organs such as the bursa of Fabricius, thymus, spleen and cecal tonsils, B lymphocyctes severely decreased or disappeared in Cy treated chickens, though no significant alteration in T cell distribution was observed.
使用多种单克隆抗体（CT3、2-6、11-39、TCR1、TCR2、TCR3、L22、11G、3E8、B-4D-4、A-13），通过免疫组织化学方法比较了环磷酰胺（Cy）处理鸡和未处理对照鸡的淋巴细胞在淋巴器官中的定位。在Cy处理鸡的哈德氏腺中，观察到T细胞增加，而在未处理对照中T细胞较少。这些增加的T细胞由CD4阳性或CD8阳性细胞组成。此外，这些T细胞用TCR2或TCR3抗体染色，少数细胞用TCR1抗体染色。在其他淋巴器官如法氏囊、胸腺、脾脏和盲肠扁桃体中，Cy处理鸡的B淋巴细胞严重减少或消失，而T细胞分布未观察到明显变化。
