12-O-十四烷酰佛波醇-13-乙酸酯对人白血病K562细胞两个亚克隆细胞表面抗原表达的影响

Effect of 12-O-tetradecanoylphorbol-13-acetate on expression of cell surface antigens in two subclones of human leukemia K562 cells.

作者信息

Sasaki D, Kosunago S, Mikami T, Matsumoto T, Suzuki M

机构信息

Department of Microbiology, Tohoku College of Pharmacy, Sendai, Japan.

出版信息

Biol Pharm Bull. 1993 Oct;16(10):1054-6. doi: 10.1248/bpb.16.1054.

DOI:10.1248/bpb.16.1054

PMID:8287040

Abstract

Two subclones (K562-L and -H) were previously isolated from K562 human leukemic cells according to hemoglobin production: K562-L was expressed in less than 5% and K562-H in more than 90% of dianisidine positive cells. 12-O-Tetradecanoylphorbol-13- acetate (TPA) suppressed the expression of the erythrocytic (glycophorin A) and myelocytic (CD11b) antigens in K562-L, but increased the expression of these antigens in K562-H. TPA increased the megakaryocytic (CD61) antigens in both cells. These findings suggest that there are distinct TPA responsible factors in K562-L and -H on the expression of the erythrocytic and myelocytic antigens.

摘要

先前根据血红蛋白产生情况从K562人白血病细胞中分离出两个亚克隆（K562-L和-H）：在不到5%的联茴香胺阳性细胞中表达K562-L，在超过90%的联茴香胺阳性细胞中表达K562-H。12-O-十四烷酰佛波醇-13-乙酸酯（TPA）抑制K562-L中红细胞（血型糖蛋白A）和髓细胞（CD11b）抗原的表达，但增加K562-H中这些抗原的表达。TPA增加了两种细胞中的巨核细胞（CD61）抗原。这些发现表明，K562-L和-H中存在不同的TPA相关因子，影响红细胞和髓细胞抗原的表达。