Shiraishi T, Uda Y
Laboratory of Health Chemistry, Niigata College of Pharmacy, Japan.
Biol Pharm Bull. 1993 Oct;16(10):956-9. doi: 10.1248/bpb.16.956.
Lignoceroyl-coenzyme A (CoA) ligase activity was detected in microsomal fractions from chicken liver in the presence of alpha-cyclodextrin as a solubilizing agent of lignoceric acid. Heptakis(2,6-di-O-methyl)-beta-cyclodextrin (dimethyl-beta-cyclodextrin) and hexakis(2,6-di-O-methyl)-alpha-cyclodextrin (dimethyl-alpha-cyclodextrin), among the cyclodextrins tested, were more effective than alpha-cyclodextrin as solubilizing agents. We have characterized lignoceroyl-CoA ligase activity in comparison with palmitoyl-CoA ligase activity. Lignoceroyl-CoA and palmitoyl-CoA ligase activities showed a similar dependency on CoA concentration. However, lignoceroyl-CoA ligase activity exhibited responses to the Mg2+ ion, adenosine triphosphate (ATP), ATP analogues (adenosine monophosphate (AMP) and adenosine diphosphate (ADP)), and heat treatment, which were distinctly different from the responses of palmitoyl-CoA ligase activity. These results are consistent with the idea that lignoceroyl-CoA and palmitoyl-CoA are synthesized by two different enzymes.
在以α-环糊精作为木质蜡酸增溶剂的情况下，在鸡肝微粒体组分中检测到了木质蜡酰辅酶A（CoA）连接酶活性。在所测试的环糊精中，七（2,6-二-O-甲基）-β-环糊精（二甲基-β-环糊精）和六（2,6-二-O-甲基）-α-环糊精（二甲基-α-环糊精）作为增溶剂比α-环糊精更有效。我们已将木质蜡酰-CoA连接酶活性与棕榈酰-CoA连接酶活性进行了表征比较。木质蜡酰-CoA和棕榈酰-CoA连接酶活性对CoA浓度表现出相似的依赖性。然而，木质蜡酰-CoA连接酶活性对Mg2+离子、三磷酸腺苷（ATP）、ATP类似物（一磷酸腺苷（AMP）和二磷酸腺苷（ADP））以及热处理的反应与棕榈酰-CoA连接酶活性的反应明显不同。这些结果与木质蜡酰-CoA和棕榈酰-CoA由两种不同酶合成的观点一致。
