Reed T J, Reid A, Wallberg K, O'Leary T J, Frizzera G
Department of Hematologic and Lymphatic Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306-6000.
Diagn Mol Pathol. 1993 Mar;2(1):42-9.
Formalin-fixed, paraffin-embedded tissue from B-cell malignant lymphomas (26), reactive lymphadenopathies (8), non-B-cell malignancies (5), and atypical lymphoproliferative lesions (7) were analyzed for clonal immunoglobulin heavy chain gene rearrangement by the polymerase chain reaction (PCR), using consensus primers for the variable and joining regions of the gene. By employing a high-resolution gel electrophoresis technique, we were able to demonstrate one or two dominant bands, indicating a clonal population, in 15 of the 23 cases (65%) of B-cell lymphoma in which amplification occurred. Six of six reactive lymph nodes in which amplification occurred produced a multi-banded pattern indicative of a polyclonal population. This improved PCR technique allows a clearer distinction between clonal and polyclonal patterns than other previously proposed methods. It also works well in paraffin-embedded tissue and may therefore be a useful adjunct to the diagnostic armamentarium applied to archival material.
采用聚合酶链反应（PCR），使用针对免疫球蛋白重链基因可变区和连接区的通用引物，对26例B细胞恶性淋巴瘤、8例反应性淋巴结病、5例非B细胞恶性肿瘤以及7例非典型淋巴增生性病变的福尔马林固定石蜡包埋组织进行克隆性免疫球蛋白重链基因重排分析。通过采用高分辨率凝胶电泳技术，我们能够在23例发生扩增的B细胞淋巴瘤中的15例（65%）中显示出一条或两条优势条带，表明存在克隆性群体。在发生扩增的6例反应性淋巴结中，有6例产生了多带模式，表明为多克隆群体。与其他先前提出的方法相比，这种改进的PCR技术能够更清晰地区分克隆性和多克隆模式。它在石蜡包埋组织中也能很好地发挥作用，因此可能是应用于存档材料的诊断手段中的一种有用辅助方法。
