Collins I, Newlon C S
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry-New Jersey Medical School, Newark.
Cell. 1994 Jan 14;76(1):65-75. doi: 10.1016/0092-8674(94)90173-2.
All recombination models postulate one or more recombination intermediates that are joint molecules containing two homologous parental molecules. A spike of branched DNA molecules not seen in DNA from mitotic cells was found in the two-dimensional gel analysis of meiotic DNA from S. cerevisiae. The mass of molecules in the spike, the timing of its appearance and disappearance, and its absence from a recombination-defective spo11 mutant are consistent with the hypothesis that it contains recombination intermediates. The spike changes in mass as predicted for joint molecules containing DNA from homologous chromosomes rather than sister chromatids in a strain heterozygous for an RFLP. Finally, joint molecules containing DNA from homologous chromosomes were not found, suggesting that the block to recombination between homologous sequences occurs prior to the formation of joint molecules.
所有重组模型都假定存在一个或多个重组中间体，这些中间体是包含两个同源亲本分子的联合分子。在酿酒酵母减数分裂DNA的二维凝胶分析中，发现了有丝分裂细胞DNA中未出现的分支DNA分子峰值。该峰值处分子的质量、其出现和消失的时间，以及在重组缺陷型spo11突变体中未出现，都与它包含重组中间体的假设一致。在一个RFLP杂合菌株中，该峰值的质量变化符合包含同源染色体而非姐妹染色单体DNA的联合分子的预测。最后，未发现包含同源染色体DNA的联合分子，这表明同源序列间重组的障碍发生在联合分子形成之前。
