Daher R, Van Lente F
Department of Chemistry, Cleveland State University, OH 44115.
Clin Chem. 1994 Jan;40(1):62-70.
We developed an assay for the direct determination of selenium in serum with a Perkin-Elmer Model 4100ZL Zeeman atomic absorption spectrometer and Ag-Cu-Mg modifier. We used this assay to analyze concanavalin A-bound selenoprotein (CABSP) in human serum after concanavalin A (ConA) affinity chromatography. The CABSP was identified as a single-chain glycoprotein of 57.3 kDa. Carbohydrate units were N- and O-linked to the protein. The selenium moiety was selenocysteine. Total selenium, glutathione peroxidase (GPX; EC 1.11.1.9), ConA-bound selenium (CABS), and alpha 1-acid glycoprotein (AAG) were determined in normal subjects and patients with various pathological conditions. CABS accounted for 44.1% +/- 6.3% of total selenium in sera from normal subjects and 46.5% +/- 3.9% to 55.1% +/- 8.1% in sera from patients with a variety of diseases. Total selenium in serum was well correlated with serum CABS (r = 0.860), but not with serum GPX activity (r = 0.117), for all patients studied. Serum CABS increased in normal subjects after selenium supplementation. Serum CABSP did not behave as an acute-phase reactant, compared with AAG.
我们使用珀金埃尔默4100ZL型塞曼原子吸收光谱仪和银 - 铜 - 镁改性剂开发了一种直接测定血清中硒的分析方法。我们使用该方法对伴刀豆球蛋白A（ConA）亲和层析后的人血清中伴刀豆球蛋白A结合硒蛋白（CABSP）进行分析。CABSP被鉴定为一种57.3 kDa的单链糖蛋白。碳水化合物单元通过N - 连接和O - 连接与蛋白质相连。硒部分为硒代半胱氨酸。在正常受试者和患有各种病理状况的患者中测定了总硒、谷胱甘肽过氧化物酶（GPX；EC 1.11.1.9）、ConA结合硒（CABS）和α1 - 酸性糖蛋白（AAG）。在正常受试者血清中，CABS占总硒的44.1%±6.3%，在患有各种疾病的患者血清中占46.5%±3.9%至55.1%±8.1%。在所研究的所有患者中，血清总硒与血清CABS高度相关（r = 0.860），但与血清GPX活性无关（r = 0.117）。补充硒后，正常受试者血清CABS增加。与AAG相比，血清CABSP不表现为急性期反应物。
