Veillon C, Patterson K Y, Nagey D A, Tehan A M
Beltsville Human Nutrition Research Center, US Department of Agriculture, MD 20705.
Clin Chem. 1994 Jan;40(1):71-3.
We used an established isotope dilution gas chromatography-mass spectrometry method to measure blood volumes with an enriched stable isotope of chromium. The results were compared with those of the conventional method, which involves radioactive 51Cr. The two methods were compared simultaneously in two male subjects of different sizes, and essentially identical volumes were obtained. The isotope dilution method is insensitive to contamination with natural (unenriched) chromium and can be used to measure all of the stable isotopes of chromium. The method has potential applications in simultaneous erythrocyte survival studies. The absence of radioactivity makes possible an accurate means of studying blood volume changes in children and during pregnancy.
我们采用一种既定的同位素稀释气相色谱-质谱分析法，用富集的稳定铬同位素来测量血容量。将结果与传统方法（涉及放射性51Cr）的结果进行比较。在两名不同体型的男性受试者中同时对这两种方法进行比较，得到的血容量基本相同。同位素稀释法对天然（未富集）铬的污染不敏感，可用于测量所有铬的稳定同位素。该方法在同步红细胞存活研究中具有潜在应用。无放射性使得准确研究儿童和孕期血容量变化成为可能。
