Ware J, Norman M, Larsson M
Res Exp Med (Berl). 1984;184(2):125-30. doi: 10.1007/BF01852359.
The method of posthaemorrhagic blood volume (BV) determination by simple haematocrit measurement has been compared with the conventional isotope dilution technique. 51Cr tagged erythrocytes and 125IHSA were used to estimate RBC volume and plasma volume in non-starved male Sprague-Dawley rats. Two series of experiments were carried out by two different investigatory groups. Haemorrhage was inflicted by 60 or 90 min of haemorrhagic hypotension at 70 mm Hg, causing 41% and 56% loss of the initial estimated BVs, respectively. There was agreement in both series for the initial blood volume indices; RBC volume, 2.82 ml x 100 g-1 b.wt.; plasma volume 3.33 ml x 100 g-1 b.wt. and F cells, 0.91. Using the RBC volume data, the calculated residual BVs after haemorrhage corresponded accurately to the isotope measurements in both series. It is concluded that non-splenectomized rats may be used for accurate BV analysis after haemorrhage if the basal data for the strain used are known.
通过简单的血细胞比容测量来测定出血后血容量（BV）的方法已与传统的同位素稀释技术进行了比较。使用51Cr标记的红细胞和125I人血清白蛋白来估计非饥饿雄性Sprague-Dawley大鼠的红细胞体积和血浆体积。两个不同的研究小组进行了两个系列的实验。通过在70毫米汞柱下进行60或90分钟的出血性低血压来造成出血，分别导致初始估计血容量损失41%和56%。两个系列的初始血容量指标一致；红细胞体积为2.82毫升×100克-1体重；血浆体积为3.33毫升×100克-1体重，F细胞为0.91。利用红细胞体积数据，两个系列中出血后计算出的残余血容量与同位素测量结果精确对应。得出的结论是，如果已知所用品系的基础数据，非脾切除大鼠可用于出血后准确的血容量分析。
