Shield P W, Halford J A, Goulden R
Pathology Department, Royal Brisbane Hospital, Herston, Australia.
Diagn Cytopathol. 1993 Oct;9(5):590-4. doi: 10.1002/dc.2840090524.
A technique for long-term storage of cytological specimens at -70 degrees C was evaluated with a range of nongynaecological cytology specimens. The effects of frozen storage on the cellularity, morphology, ultrastructure, and reactivity to cytochemical and immunochemical stains were investigated. These parameters were compared in preparations made from specimens processed conventionally and after frozen storage. No significant deterioration in the quality of subsequent preparations was apparent after storage. The method is technically simple and has proven successful for storing a range of specimens, including serous effusions, urine samples, and fine-needle aspirates. This technique allows storage of material until initial microscopic evaluation is complete. If required, the relevant adjuvant procedure, such as cell blocking, may then be carried out using the stored material. Wastage of cytological material on unnecessary preparations is minimised, and material may be stored over years for research or confirmation procedures.
采用一系列非妇科细胞学标本评估了在-70℃下长期保存细胞学标本的技术。研究了冷冻保存对细胞数量、形态、超微结构以及对细胞化学和免疫化学染色反应性的影响。将这些参数在传统处理的标本和冷冻保存后的标本制备物中进行比较。保存后后续制备物的质量没有明显恶化。该方法技术简单，已证明可成功保存一系列标本，包括浆液性积液、尿液样本和细针穿刺抽吸物。该技术允许在初步显微镜评估完成之前保存材料。如果需要，然后可以使用保存的材料进行相关的辅助程序，如细胞块制作。将不必要制备过程中细胞学材料的浪费降至最低，并且材料可以保存数年用于研究或确认程序。
