Leibiger I B, Walther R, Pett U, Leibiger B
Institut für Biochemie, Klinikum, Medizinische Fakultät, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
FEBS Lett. 1994 Jan 10;337(2):161-6. doi: 10.1016/0014-5793(94)80265-3.
Nested deletion mutants of the 5' flanking region of the beta-cell transcription unit of the rat glucokinase gene (r beta GK) were fused to the CAT-reporter gene. Transient expression studies in HIT M2.2.2 and BHK21 cells revealed a distal (upstream of -359) and a proximal promoter region (between -278/-49) harbouring positive and negative regulatory elements. DNaseI footprinting revealed three protected areas between nucleotides -190 and -60. DNA-elements playing a crucial role in transcriptional control of the insulin genes (IEB- and CT-motifs) have been detected within the proximal promoter region and contribute to beta-cell specific gene regulation. 3' deletion analysis revealed that DNA-elements located downstream from transcription initiation sites (up to +123) contribute to transcriptional regulation.
将大鼠葡萄糖激酶基因（rβGK）β细胞转录单元5'侧翼区的嵌套缺失突变体与CAT报告基因融合。在HIT M2.2.2和BHK21细胞中进行的瞬时表达研究显示，存在一个远端（-359上游）和一个近端启动子区域（-278/-49之间），分别含有正调控元件和负调控元件。DNaseI足迹分析显示在核苷酸-190至-60之间有三个受保护区域。在近端启动子区域内检测到了在胰岛素基因转录调控中起关键作用的DNA元件（IEB基序和CT基序），并参与β细胞特异性基因调控。3'缺失分析表明，位于转录起始位点下游（直至+123）的DNA元件有助于转录调控。
