Schwarz U, Wunderlich G, Brossmer R
Institut für Analytische Chemie, Technische Universität Dresden, Germany.
FEBS Lett. 1994 Jan 10;337(2):213-6. doi: 10.1016/0014-5793(94)80275-0.
This paper presents a new method for site-specific labelling of antibodies employing enzymatic reactions without oxidizing or reducing agents. IgG was first treated with immobilized sialidase from Clostridium perfringens to cleave bound NeuAc. CMP-9-deoxy-9-salizoyl-NeuAc, an activated sialic acid analogue, was labelled with 131I via the iodogen-method in high yields (> 95%). Then the oligosaccharide chains of antibodies were labelled yield with the radioactive NeuAc analogue by transfer using alpha-2,6-sialyltransferase from rat liver in 50%.
本文提出了一种在不使用氧化剂或还原剂的情况下，利用酶促反应对抗体进行位点特异性标记的新方法。首先用来自产气荚膜梭菌的固定化唾液酸酶处理IgG，以切割结合的NeuAc。通过碘代法以高产率（>95%）用131I标记CMP-9-脱氧-9-水杨酰基-NeuAc，一种活化的唾液酸类似物。然后，使用大鼠肝脏中的α-2,6-唾液酸转移酶通过转移以50%的产率用放射性NeuAc类似物标记抗体的寡糖链。
