Monoclonal antibodies against the plant cytokinin, cis-zeatin riboside.

Three monoclonal antibodies (two IgG1 and one IgA) prepared against the plant cytokinin, cis-zeatin riboside, were characterized for use in a fluorescence competition enzyme linked immunosorbant assay (F-ELISA). Immunoassays that employed each antibody detected femtomole quantities of the homologous cytokinin and characteristic quantities of structurally-related cytokinins. One of the antibodies was used to quantify HPLC-purified cis-zeatin riboside recovered from spiked samples of wheat leaves. These are the first monoclonal antibodies against a cis- derivative of a plant cytokinin and will be useful for purification and quantification of cis-zeatin and the 9-riboside and 9-glucoside in plant samples.