Tournaye H, Van der Linden M, Van den Abbeel E, Devroey P, Van Steirteghem A
Centre for Reproductive Medicine, University Hospital, Vrije Universiteit Brussel, Belgium.
Hum Reprod. 1993 Nov;8(11):1948-54. doi: 10.1093/oxfordjournals.humrep.a137966.
Previous work from our laboratory has revealed that brief exposure of mouse zygotes to 3.6 and 7.2 mM pentoxifylline reduced cell numbers in mouse embryos reaching the blastocyst stage in vitro. Since a decrease in cell numbers may impair further development, the present study aimed to assess the implantation in vitro and the development beyond implantation of these embryos. Blastocysts cultured from the one-cell stage in the presence of 5, 10 and 50 microM pentoxifylline, i.e. concentrations expected in the secretions of the genital tract after oral administration, were shown to develop normally to the egg cylinder stage in vitro. Blastocysts, obtained after culture in vitro of zygotes exposed approximately 29 h after human chorionic gonadotrophin (HCG) for 30 min to 3.6 and 7.2 mM pentoxifylline, i.e. the concentrations used for enhancing sperm function in vitro, could implant but their development to the egg cylinder stage was found to be impaired. The same effect was noticed if blastocysts, grown in vitro from the one-cell stage, were exposed to 3.6 and 7.2 mM pentoxifylline for 30 min in vitro approximately 120 h after HCG. These findings imply that when pentoxifylline is used to enhance sperm function in vitro, it should be washed out of the preparation used for insemination.
我们实验室之前的研究表明，将小鼠受精卵短暂暴露于3.6和7.2 mM的己酮可可碱中，会减少体外发育至囊胚阶段的小鼠胚胎中的细胞数量。由于细胞数量减少可能会损害进一步发育，因此本研究旨在评估这些胚胎的体外着床以及着床后的发育情况。在5、10和50 microM己酮可可碱存在的情况下，从单细胞阶段开始培养的囊胚，即口服给药后生殖道分泌物中预期的浓度，在体外能正常发育至卵圆柱阶段。在人绒毛膜促性腺激素（HCG）注射约29小时后，将受精卵暴露于3.6和7.2 mM己酮可可碱中30分钟（即用于体外增强精子功能的浓度），然后进行体外培养，所获得的囊胚能够着床，但发现它们发育至卵圆柱阶段受到损害。如果从单细胞阶段开始在体外培养的囊胚在HCG注射约120小时后在体外暴露于3.6和7.2 mM己酮可可碱中30分钟，也会观察到相同的效果。这些发现表明，当使用己酮可可碱在体外增强精子功能时，应将其从用于授精的制剂中冲洗掉。
