Ngassapa O, Beecher C W, Pezzuto J M, Farnsworth N R, Henderson T O, Boye G L
Progam for Collaborative Research in the Pharmaceutical Sciences, College of Pharmacy, University of Illinois at Chicago 60680.
J Nat Prod. 1993 Nov;56(11):1872-7. doi: 10.1021/np50101a002.
A known triterpene glycoside, 3-O-[beta-D-glucopyranosyl-(1"-6')-2'-acetamido-2'-deoxy-beta-D-gluco pyranosyl]olean-12-en-28-oic acid [3], and new sulfated triterpene, echinocystic acid-3-O-sodium sulfate [4], have been isolated from the stem bark of Tetrapleura tetraptera. Compound 3 was 100% lethal to Biomphalaria glabrata at 20 ppm, while 4 was not molluscicidal at the same concentration. In a forward mutation assay utilizing Salmonella typhimurium strain TM677, T. tetraptera stem bark extracts were found to be mutagenic in the absence of a metabolic activating system (S-9). An MeOH extract of the fruit exhibited weak mutagenic activity only in the presence of S-9. The stem bark isolates, which included aridanin [1], 3-O-(2'-acetamido-2'-deoxy-beta-D-glucopyranosyl)echinocystic acid [2], and compounds 3 and 4, were not mutagenic either with or without metabolic activation.
从四翅四棱豆的茎皮中分离出一种已知的三萜糖苷，即3 - O - [β - D - 吡喃葡萄糖基 - (1″ - 6′) - 2′ - 乙酰氨基 - 2′ - 脱氧 - β - D - 吡喃葡萄糖基]齐墩果 - 12 - 烯 - 28 - 酸[3]，以及一种新的硫酸化三萜，即刺囊酸 - 3 - O - 硫酸钠[4]。化合物3在20 ppm浓度下对光滑双脐螺的致死率为100%，而化合物4在相同浓度下没有杀螺活性。在利用鼠伤寒沙门氏菌TM677菌株进行的正向突变试验中，发现四翅四棱豆茎皮提取物在没有代谢激活系统（S - 9）的情况下具有致突变性。果实的甲醇提取物仅在存在S - 9时表现出微弱的致突变活性。茎皮分离物，包括阿里丹宁[1]、3 - O - (2′ - 乙酰氨基 - 2′ - 脱氧 - β - D - 吡喃葡萄糖基)刺囊酸[2]以及化合物3和4，无论有无代谢激活都没有致突变性。
