Mega M, Ongaro G, Paternoster D, Carretti N, De Laurentiis G
Quad Sclavo Diagn. 1976 Sep;12(3):258-77.
On 90 samples of amniotic fluid coming from 54 cases of Rh-isoimmunization submitted to amniocentesis one or more times, the following tests were done: 1.) Coombs indirect test; 2) immunoelectrophoresis; 3) quantitative determination of immunoglobulins. The results were related to the degree of immunization as determined by Coombs indirect test on the mothers' serum and spectrophotometric curve of the amniotic fluid. For reasons of comparison, quantitative determination of immunoglobulins were performed on samples of amniotic fluid from 39 pregnant non-immunized patients. It was found that there was a direct relationship between antibody titers in the mothers' serum and those in the amniotic fluid, with the latter values always being inferior. Moreover, it was found that the level of IgG in the amniotic fluid and the degree of maternal immunization were proportional, with higher titers in the more severe cases. In addition to the routine tests, the determination of anti-Rh antibodies and titers of IgG in amniotic fluid can be useful in further evaluating degree of immunization in Rh-incompatibility. Furthermore, small quantities of IgA were found in amniotic fluid: these IgA were probably of secretory type (SIgA) and of amniotic origin, and therefore independent of any active immunization. Finally, all determinations of IgM were negative.
对来自54例接受过一次或多次羊膜腔穿刺术的Rh血型免疫孕妇的90份羊水样本进行了以下检测：1.）库姆斯间接试验；2）免疫电泳；3）免疫球蛋白定量测定。结果与通过对母亲血清进行库姆斯间接试验和羊水分光光度曲线确定的免疫程度相关。为了进行比较，对39例未免疫的孕妇羊水样本进行了免疫球蛋白定量测定。发现母亲血清中的抗体滴度与羊水中的抗体滴度之间存在直接关系，后者的值总是较低。此外，发现羊水中IgG的水平与母亲的免疫程度成正比，病情越严重，滴度越高。除了常规检测外，羊水抗Rh抗体和IgG滴度的测定有助于进一步评估Rh血型不合中的免疫程度。此外，在羊水中发现了少量IgA：这些IgA可能是分泌型（SIgA）且源自羊水，因此与任何主动免疫无关。最后，所有IgM的测定均为阴性。
