Surynek J, Kucera A, Janů J
Vet Med (Praha). 1976 Nov;21(11):675-82.
A trial was performed in 204 healthy calves (heifers) of the Bohemian Spotted breed in the post-natal period from birth to the age of four months. The activities of the following enzymes in blood plasma were determined: L-aspartate: 2-oxoglutarate: aminotransferase, EC.2.6.1.1. (GOT), L-alanine: oxoglutarate: aminotransferase, EC.2.6.1.2. (GPT), L-lactate: NAD oxidoreductase, EC.1.1.1.27 (LDH), and orthophosphoric acid monoester phosphohydrolase, EC.3.1.3.1. (alkaline phosphatase). The calves were divided into age categories according to the date of birth with an interval of one week. GOT activity in blood plasma increased significantly until the age of eight weeks (from the original value of 1.1708 +/- 0.2598 micronmol ml-1 to 1.8150 +/- 0.6362 micronmol ml-1, with the maximum of 2.0317 +/- 0.7777 micronmol ml-1 of plasma in the sixth week). In the subsequent period the GOT curve has not a characteristic course. While the activity of GOT increased in the first weeks after birth, the activity of GPT showed a significant drop (from the original level of 0.9000 +/- 0.3364 micronmol ml-1 to the minimum of 0.3675 +/- 0.1901 micronmol ml-1 of plasma in the seventh week); from the 10th week on the values rise so that at the end of the period of study they reach almost the same levels as in calves in the first postnatal week. The activity of LDH in blood plasma remains at almost the same level in the first five weeks after birth (between 43.4025 +/- 8.4893 micronmol ml-1 and 46.3792 +/- 14.8952 micronmol ml-1 of plasma); it was at a statistically significantly higher level only in a short period between the 7th and 10th week after birth. The highest values of alkaline phosphatase in blood plasma were recorded at the age of two or three weeks (maximum in the second week 23.9833 +/- 9.0945 micronmol ml-1 of plasma); from the fourth week on, the values of alkaline phosphatase are significantly lower until the end of the test period, ranging betweek 5.3133 +/- 1.6017 micronmol ml-1 and 7.5425 +/- 2.2437 micronmol ml-1 of plasma. Changes conditioned by postnatal development were observed in the development of all the enzymatic activities under study, the greatest changes being observed in alkaline phosphatase.
对204头波希米亚斑点品种的健康犊牛（小母牛）在出生后至4月龄的产后阶段进行了一项试验。测定了血浆中以下几种酶的活性：L-天冬氨酸：2-氧代戊二酸：氨基转移酶，EC.2.6.1.1.（谷草转氨酶）；L-丙氨酸：氧代戊二酸：氨基转移酶，EC.2.6.1.2.（谷丙转氨酶）；L-乳酸：NAD氧化还原酶，EC.1.1.1.27（乳酸脱氢酶）；以及正磷酸单酯磷酸水解酶，EC.3.1.3.1.（碱性磷酸酶）。根据出生日期将犊牛分为不同年龄组，间隔为一周。血浆中谷草转氨酶的活性在8周龄前显著升高（从最初值1.1708±0.2598微摩尔/毫升升至1.8150±0.6362微摩尔/毫升，在第6周血浆中最高值为2.0317±0.7777微摩尔/毫升）。在随后的时期，谷草转氨酶曲线没有特征性变化。虽然出生后的头几周谷草转氨酶活性增加，但谷丙转氨酶活性显著下降（从最初水平0.9000±0.3364微摩尔/毫升降至第7周血浆中的最低值0.3675±0.1901微摩尔/毫升）；从第10周起，数值上升，以至于在研究期末它们几乎达到与出生后第一周犊牛相同的水平。出生后前5周血浆中乳酸脱氢酶的活性几乎保持在同一水平（血浆中为43.4025±8.4893微摩尔/毫升至46.3792±14.8952微摩尔/毫升之间）；仅在出生后第7周和第10周之间的短时间内其水平在统计学上显著较高。血浆中碱性磷酸酶的最高值记录在2或3周龄时（第2周最高，血浆中为23.9833±9.0945微摩尔/毫升）；从第4周起，碱性磷酸酶的值显著降低，直到试验期结束，血浆中其值在5.3133±1.6017微摩尔/毫升至7.5425±2.2437微摩尔/毫升之间。在所研究的所有酶活性的发展过程中都观察到了由产后发育引起的变化，其中碱性磷酸酶的变化最为明显。
