Brazhnik E S, Vinogradova O S, Stafekhina V S, Kichigina V F
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino.
Neurosci Behav Physiol. 1993 Nov-Dec;23(6):510-9. doi: 10.1007/BF01153677.
A statistical analysis of the baseline activity of neurons, recorded intracellularly in the hippocampus of awake, nonimmobilized rabbits in three states, control and during the systemic administration of eserine and scopolamine, was carried out. Neurons of the hippocampus were additionally tested in a similar manner following the chronic basal undercutting of the septum, removing stem influences. The cholinergic substances regulate the number of neurons of the hippocampus having theta modulation and the degree of its stability, but do not influence its frequency. When the cholinergic theta rhythm is activated, regularization of the activity takes place with the suppression of delta modulation and of "complex spikes"; its blockade is accompanied by the opposite changes. Both substances stably alter the level of the baseline frequency of discharges of the majority of neurons, although the total average frequency remains constant. Regression analysis shows the predominance of a decrease in the activity in high-frequency (> 25 spikes/sec) and an increase in the low-frequency (< 25 spikes/sec) neurons during the effect of both substances. The constancy of the total average frequency and the unidirectionality of the shifts in the level of discharges of the neurons during the intensification (eserine) and blockade (scopolamine) of the cholinergic component of the theta rhythm points to the fact that the cholinergic septal input directly influences mainly the structure but not the level of the activity of the hippocampal neurons.
对清醒、未固定的兔子海马体中细胞内记录的神经元基线活动进行了统计分析，实验分三种状态：对照状态以及全身给予毒扁豆碱和东莨菪碱期间。在对隔膜进行慢性基底切断以消除其影响后，以类似方式对海马体神经元进行了额外测试。胆碱能物质调节具有θ调制的海马体神经元数量及其稳定性程度，但不影响其频率。当胆碱能θ节律被激活时，活动会发生规则化，同时δ调制和“复合尖峰”受到抑制；其阻断则伴随着相反的变化。尽管总平均频率保持不变，但两种物质均稳定地改变了大多数神经元放电基线频率的水平。回归分析表明，在两种物质的作用下，高频（>25次/秒）神经元活动减少，低频（<25次/秒）神经元活动增加占主导。在θ节律胆碱能成分增强（毒扁豆碱）和阻断（东莨菪碱）期间，总平均频率的恒定以及神经元放电水平变化的单向性表明，胆碱能隔区输入主要直接影响海马体神经元活动的结构而非水平。
