Crowe A J, McGlade J, Pawson T, Hayman M J
Department of Microbiology, State University of New York, Stony Brook 11794-5222.
Oncogene. 1994 Feb;9(2):537-44.
The S13 avian erythroblastosis viral genome encodes an oncogenic tyrosine kinase, termed env-sea, that is capable of transforming fibroblasts and erythroblasts. Although the tyrosine kinase activity of the env-sea protein has been shown to be necessary for transformation, no substrates for this enzyme have been detected in vivo. Here we demonstrate that the recently described shc proteins are phosphorylated on tyrosine residues in both S13 transformed fibroblasts and erythroblasts. Furthermore, using an S13 temperature sensitive mutant, we show that the phosphorylation of the shc proteins occurs concomitantly with the activation of the tyrosine kinase activity of the env-sea protein. These observations make the phosphorylation of the shc proteins a good candidate for being involved in oncogenic signaling by the env-sea oncoprotein.
S13禽成红细胞增多症病毒基因组编码一种致癌性酪氨酸激酶，称为env-sea，它能够转化成纤维细胞和红细胞。尽管已证明env-sea蛋白的酪氨酸激酶活性对于转化是必需的，但在体内尚未检测到该酶的底物。在这里，我们证明最近描述的shc蛋白在S13转化的成纤维细胞和红细胞中的酪氨酸残基上被磷酸化。此外，使用S13温度敏感突变体，我们表明shc蛋白的磷酸化与env-sea蛋白酪氨酸激酶活性的激活同时发生。这些观察结果使shc蛋白的磷酸化成为参与env-sea癌蛋白致癌信号传导的良好候选者。
