Morpurgo E, Rigotti P, Capalbo M, Baldan N, Pittoni G, Valente M L, Bindoli A, Ancona E
University of Padua, Institute of General Surgery II, Italy.
Ren Fail. 1993;15(5):581-5. doi: 10.3109/08860229309069407.
This study evaluated renal function and histological and oxidative injury in transplanted kidneys harvested after prolonged warm ischemia in pigs. In 8 donor pigs, kidneys were perfused in situ 120 min after cardiac arrest. One of each pair of kidneys was used for histological and biochemical studies while the other was transplanted into a recipient undergoing bilateral nephrectomy. In 6 cases, renal function was satisfactory 14 days after transplantation. Histologically, a reversible acute tubular necrosis was observed with partial recovery at the 14th postoperative day. A moderate oxidation was revealed by decreased glutathione and increased malondialdehyde levels. In spite of this ischemic injury, these findings suggest that kidneys harvested after prolonged warm ischemia can still recover after transplantation, and that non-heart-beating donors may be considered as an alternative organ source for kidney transplantation.
本研究评估了猪长时间热缺血后获取的移植肾的肾功能、组织学及氧化损伤情况。在8只供体猪中，心脏骤停120分钟后对肾脏进行原位灌注。每对肾脏中的一个用于组织学和生化研究，另一个移植到接受双侧肾切除术的受体体内。6例中，移植后14天肾功能良好。组织学上，观察到可逆性急性肾小管坏死，术后第14天部分恢复。谷胱甘肽水平降低和丙二醛水平升高显示有中度氧化。尽管存在这种缺血性损伤，但这些发现表明，长时间热缺血后获取的肾脏移植后仍可恢复，非心跳供体可被视为肾移植的替代器官来源。
