Cockcroft S, Thomas G M, Fensome A, Geny B, Cunningham E, Gout I, Hiles I, Totty N F, Truong O, Hsuan J J
Department of Physiology, University College London, United Kingdom.
Science. 1994 Jan 28;263(5146):523-6. doi: 10.1126/science.8290961.
Activation of the phospholipase D (PLD) pathway is a widespread response when cells are activated by agonists that bind receptors on the cell surface. A 16-kD cytosolic component can reconstitute guanosine triphosphate (GTP)-mediated activation of phospholipase D in HL60 cells depleted of their cytosol by permeabilization. This factor was purified and identified as two small GTP-binding proteins, ARF1 and ARF3. Recombinant ARF1 substituted for purified ARF proteins in the reconstitution assay. These results indicate that phospholipase D is a downstream effector of ARF1 and ARF3. The well-established role of ARF in vesicular traffic would suggest that alterations in lipid content by PLD are an important determinant in vesicular dynamics.
当细胞被结合于细胞表面受体的激动剂激活时，磷脂酶D（PLD）途径的激活是一种广泛存在的反应。一种16-kD的胞质成分能够在通过透化作用使其胞质耗尽的HL60细胞中，重建鸟苷三磷酸（GTP）介导的磷脂酶D激活。该因子被纯化并鉴定为两种小GTP结合蛋白，即ARF1和ARF3。重组ARF1在重建试验中替代了纯化的ARF蛋白。这些结果表明磷脂酶D是ARF1和ARF3的下游效应器。ARF在囊泡运输中已明确的作用提示，PLD引起的脂质含量改变是囊泡动力学的一个重要决定因素。
