Ansar Ahmed S, Furr M, Chickering W R, Sriranganathan N, Sponenberg D P
Department of Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061-0442.
Vet Immunol Immunopathol. 1993 Oct;38(3-4):229-39. doi: 10.1016/0165-2427(93)90083-g.
Immunological, clinical, and pathological investigations were conducted on a horse with lymphosarcoma. The immunological status was investigated by measuring the level of antibodies by single radial immunodiffusion test and the ability of lymphocytes to proliferate in response to mitogens. Multiple immunological abnormalities were noted in this horse. They were; (1) decreased IgM, IgG, and IgA levels in the serum despite hyperproteinemia; (2) increased in-vitro spontaneous lymphoproliferation which reflects augmented mitosis; (3) decreased lymphoproliferative response to T cell stimulants (e.g. Concanavalin-A (Con-A)) suggesting impaired T cell activation; (4) presence of immunosuppressive factors in serum as demonstrated by in-vitro lymphocyte culture systems. Clinical pathology findings revealed an unusual monoclonal alpha peak in the serum and morphologically abnormal lymphocytes distributed throughout the body. Serum fractionated by fast protein liquid chromatography (FPLC) revealed that the immunosuppressive factors were found in this abnormal alpha peak. The immunopathological findings in this horse are discussed.
对一匹患有淋巴肉瘤的马进行了免疫学、临床和病理学研究。通过单向免疫扩散试验测量抗体水平以及淋巴细胞对有丝分裂原的增殖能力来研究免疫状态。在这匹马身上发现了多种免疫异常。它们是：(1) 尽管存在高蛋白血症，但血清中 IgM、IgG 和 IgA 水平降低；(2) 体外自发淋巴细胞增殖增加，这反映了有丝分裂增强；(3) 对 T 细胞刺激剂（如刀豆球蛋白 A（Con-A））的淋巴细胞增殖反应降低，提示 T 细胞活化受损；(4) 体外淋巴细胞培养系统证明血清中存在免疫抑制因子。临床病理检查结果显示血清中出现异常的单克隆α峰，且形态异常的淋巴细胞分布于全身。通过快速蛋白质液相色谱（FPLC）对血清进行分级分离显示，免疫抑制因子存在于这个异常的α峰中。本文讨论了这匹马的免疫病理学发现。
