柯萨奇病毒和鼻病毒的2A蛋白酶通过一个共同的识别基序切割源自真核起始因子4γ的肽段。

2A proteinases of coxsackie- and rhinovirus cleave peptides derived from eIF-4 gamma via a common recognition motif.

作者信息

Sommergruber W, Ahorn H, Klump H, Seipelt J, Zoephel A, Fessl F, Krystek E, Blaas D, Kuechler E, Liebig H D

机构信息

BENDER+CO Ges mbH, Ernst Boehringer Institut fuer Arzneimittelforschung, Vienna, Austria.

出版信息

Virology. 1994 Feb;198(2):741-5. doi: 10.1006/viro.1994.1089.

DOI:10.1006/viro.1994.1089

PMID:8291255

Abstract

The cleavage specificities of the 2A proteinases from coxsackievirus B4 (CVB4) and human rhinovirus 2 (HRV2) on oligopeptide substrates have been determined. Comparison of the specificity of CVB4 2A proteinase with that of HRV2 2A proteinase allowed cleavable peptides to be designed using the common motif IIe/Leu-X-Thr-X*Gly; little resemblance to the viral cleavage site remained. The data also allowed the prediction of three possible cleavage sites for 2A proteinases on eIF-4 gamma; two peptides derived from these sequences were cleaved by both 2A proteinases. One of these peptides corresponds to the cleavage site for 2A proteinases mapped on eIF-4 gamma [B. J. Lamphear et al. (1993) J. Biol. Chem. 268, 19200-19203]. This supports the hypothesis that cleavage of eIF-4 gamma by picornaviral 2A proteinases occurs directly.

摘要

已确定柯萨奇病毒B4（CVB4）和人鼻病毒2（HRV2）的2A蛋白酶对寡肽底物的切割特异性。将CVB4 2A蛋白酶的特异性与HRV2 2A蛋白酶的特异性进行比较，使得可以使用共同基序Ile/Leu-X-Thr-X*Gly设计可切割的肽；与病毒切割位点的相似性所剩无几。这些数据还使得能够预测2A蛋白酶在真核翻译起始因子4γ（eIF-4γ）上的三个可能切割位点；源自这些序列的两个肽被两种2A蛋白酶切割。这些肽中的一个对应于定位在eIF-4γ上的2A蛋白酶的切割位点[B. J. 兰菲尔等人（1993年）《生物化学杂志》268，19200 - 19203]。这支持了小核糖核酸病毒2A蛋白酶对eIF-4γ的切割直接发生的假说。