Garcia-Peláez I, Arteaga M
Department of Histology, Facultad de Medicina U.N.A.M., México, D.F.
Anat Rec. 1993 Nov;237(3):378-84. doi: 10.1002/ar.1092370311.
The time of appearance of the truncus arteriosus was studied in the chick embryo using an in ovo labeling technique. Three hundred embryos at stages 13-18 of Hamburger and Hamilton were selectively labeled at the distal end of the heart tube, using gelatine-india ink label; 122 of these embryos were reincubated and 111 of them reached stages 25-28. In these stages the final location of the label was determined. Only 95 of these embryos showed both a normal heart and a label located in it. The remaining embryos were discarded due to abnormal cardiac morphology or because the label was not found. Embryos labeled at stages 13-14 had label in the conus in 42.8% of the cases and in the boundary between the conus and the truncus arteriosus in 57.1% of the cases. Label placed at stages 15-16 was located in the conus in 6.1% of the cases, in the boundary between the conus and the truncus arteriosus in 44.8% of the cases, and in the truncus arteriosus in 48.9% of the cases. Finally, label placed at stages 17-18 was located in the boundary between the conus and the truncus arteriosus in 18.7% of the cases and in the truncus arteriosus in 81.2% of the cases. Our results permit us to conclude that the truncus arteriosus appears in the chick embryo as early as stages 15-16 of Hamburger and Hamilton (50-56 hours of incubation).
利用卵内标记技术，对鸡胚动脉干出现的时间进行了研究。在汉堡和汉密尔顿第13 - 18阶段的300个胚胎中，使用明胶 - 印度墨水标记法在心脏管远端进行选择性标记；其中122个胚胎重新孵化，111个胚胎发育到第25 - 28阶段。在这些阶段确定标记的最终位置。这些胚胎中只有95个心脏正常且标记位于心脏内。其余胚胎因心脏形态异常或未找到标记而被丢弃。在第13 - 14阶段标记的胚胎中，42.8%的情况下标记位于圆锥部，57.1%的情况下标记位于圆锥部与动脉干之间的边界处。在第15 - 16阶段放置的标记，6.1%的情况下位于圆锥部，44.8%的情况下位于圆锥部与动脉干之间的边界处，48.9%的情况下位于动脉干。最后，在第17 - 18阶段放置的标记，18.7%的情况下位于圆锥部与动脉干之间的边界处，81.2%的情况下位于动脉干。我们的结果使我们能够得出结论，动脉干最早在汉堡和汉密尔顿第15 - 16阶段（孵化50 - 56小时）出现在鸡胚中。
