Mazurek N, Megidish T, Hakomori S, Igarashi Y
Biomembrane Institute, Seattle, WA 98119.
Biochem Biophys Res Commun. 1994 Jan 14;198(1):1-9. doi: 10.1006/bbrc.1994.1001.
Sphingosine-1-phosphate (Sph-1-P) has been implicated as a second messenger in control of cell motility and proliferation (e.g., Sadahira Y, et al., PNAS 89:9686, 1992; Olivera A & Spiegel S, Nature 365:557, 1993). The control mechanism for its synthesis, as catalyzed by sphingosine kinase, is crucial in signal transduction. Synthesis of Sph-1-P in Balb/c 3T3 fibroblasts (A31 variant) is strongly up-regulated by brief treatment of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA). Level of Sph-1-P in PKC-depleted cells is 10-fold higher than in undepleted cells, and a further 5-fold increase occurs after treatment with TPA. In Swiss 3T3 and B16 melanoma cells, Sph-1-P level was unaffected by TPA treatment. Thus, the effect of TPA on Sph-1-P synthesis appears to be cell type-specific.
鞘氨醇-1-磷酸（Sph-1-P）被认为是控制细胞运动和增殖的第二信使（例如，Sadahira Y等人，《美国国家科学院院刊》89:9686，1992；Olivera A和Spiegel S，《自然》365:557，1993）。由鞘氨醇激酶催化的其合成控制机制在信号转导中至关重要。用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）短暂处理细胞后，Balb/c 3T3成纤维细胞（A31变体）中Sph-1-P的合成强烈上调。PKC缺失细胞中Sph-1-P的水平比未缺失细胞高10倍，用TPA处理后进一步增加5倍。在瑞士3T3细胞和B16黑色素瘤细胞中，TPA处理对Sph-1-P水平没有影响。因此，TPA对Sph-1-P合成的影响似乎具有细胞类型特异性。
