rabphilin-3A（一种Rab3A的假定靶蛋白）在PC12细胞中钙依赖性胞吐作用位点的定位。

Localization of rabphilin-3A, a putative target protein for Rab3A, at the sites of Ca(2+)-dependent exocytosis in PC12 cells.

作者信息

Wada K, Mizoguchi A, Kaibuchi K, Shirataki H, Ide C, Takai Y

机构信息

Department of Biochemistry, Kobe University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1994 Jan 14;198(1):158-65. doi: 10.1006/bbrc.1994.1023.

DOI:10.1006/bbrc.1994.1023

PMID:8292018

Abstract

Rab3A/Smg 25A, a small GTP-binding protein, is highly concentrated in presynapse of neurons and implicated in neurotransmitter release. We have recently identified a putative target protein for Rab3A, isolated its cDNA, and designated it as Rabphilin-3A. To examine whether Rabphilin-3A as well as Rab3A is localized at the sites of Ca(2+)-dependent exocytosis, we investigated here localization of Rabphilin-3A and Rab3A in comparison with the sites of exocytosis in the differentiated PC12 cells. Rabphilin-3A as well as Rab3A was highly concentrated at the tips of the neurites where Ca(2+)-dependent exocytosis took place. Inversely, neither Rabphilin-3A nor Rab3A was concentrated at the tips of the neurites where Ca(2+)-dependent exocytosis did not take place. These results suggest that Rabphilin-3A as well as Rab3A constitutes a part of the machinery necessary for neurotransmitter release.

摘要

Rab3A/Smg 25A是一种小GTP结合蛋白，高度集中于神经元的突触前，与神经递质释放有关。我们最近鉴定出Rab3A的一个假定靶蛋白，分离出其cDNA，并将其命名为Rabphilin-3A。为了研究Rabphilin-3A以及Rab3A是否定位于Ca(2+)依赖性胞吐作用的位点，我们在此研究了Rabphilin-3A和Rab3A在分化的PC12细胞中的定位，并与胞吐作用位点进行了比较。Rabphilin-3A以及Rab3A高度集中于发生Ca(2+)依赖性胞吐作用的神经突尖端。相反，在不发生Ca(2+)依赖性胞吐作用的神经突尖端，Rabphilin-3A和Rab3A均未集中。这些结果表明，Rabphilin-3A以及Rab3A构成了神经递质释放所需机制的一部分。