Canning E U, Sinden R E, Landau I, Miltgen F
Ann Parasitol Hum Comp. 1976 Nov-Dec;51(6):607-23. doi: 10.1051/parasite/1976516607.
An immature merocyst of Hepatocystis malayensis and gametocytes of H. brayi were studied with the electron microscope. The merocyst consisted of a highly complex cytoplasmic reticulum ramifying through an amorphous matrix: the entire complex was enclosed by a simple unit membrane. The host cell was apparently destroyed completely during growth of the cyst. Immature gametocytes were highly amoeboid and showed extensive vacuolisation or attenuation of the cytoplasm. The nucleus contained one or two prominent nucleoli. Mature gametocytes had compact cytoplasm and contained pyriform osmiophilic bodies which were believed to function in the release of the parasites from the host cells. Macrogametocytes were distinguished from microgametocytes by cytoplasmic differences in numbers of ribosomes, and cristate mitochondria and in the extent of development of the smooth endoplasmic reticulum. The compact nuclei of the macrogametocytes had inconspicuous DNA but prominent nucleoli whereas those of the microgametocytes were irregular and showed a central aggregate of DNA. In microgametogenesis karyokinesis of the parent nucleus was delayed until axoneme formation was complete. Then the nuclear buds were extruded into emerging microgametes. At fertilisation the plasmalemmas of the two gametes fused and the single axoneme and nucleus of the microgamete moved into the cytoplasm of the macrogamete.
利用电子显微镜对马来肝囊原虫的未成熟裂殖体和布氏肝囊原虫的配子体进行了研究。裂殖体由高度复杂的细胞质网组成，这些细胞质网在无定形基质中分支：整个复合体被一层简单的单位膜包围。在囊肿生长过程中，宿主细胞显然被完全破坏。未成熟的配子体高度变形虫状，细胞质出现广泛的空泡化或萎缩。细胞核含有一个或两个明显的核仁。成熟的配子体细胞质致密，含有梨形嗜锇体，据信其功能是将寄生虫从宿主细胞中释放出来。大配子体与小配子体的区别在于核糖体数量、嵴状线粒体以及滑面内质网发育程度的细胞质差异。大配子体致密的细胞核中DNA不明显，但核仁突出，而小配子体的细胞核不规则，显示DNA集中在中央。在小配子发生过程中，亲代细胞核的核分裂延迟到轴丝形成完成。然后核芽被挤出形成新的小配子。受精时，两个配子的质膜融合，小配子的单个轴丝和细胞核移入大配子的细胞质中。
