Torres A R, Burger R, Rich A, Spendlove R, Warren R P
HyClone Laboratories, Logan, UT.
Biotechniques. 1993 Dec;15(6):1106-9.
The proliferation and activation of murine and human T-lymphocytes in a high-protein lymphocyte culture fluid (LCF) is compared to that of cells cultured in 10% fetal bovine serum (FBS). Tumor-infiltrating lymphocytes (TIL) proliferate exponentially in the LCF for up to 46 days and generate cell numbers that are nearly 100,000-fold greater than cells cultured in FBS. This rapid growth of T cells in LCF could have an important impact in adoptive immunotherapy and gene therapy since cell growth is a limiting factor in these technologies.
将小鼠和人类T淋巴细胞在高蛋白淋巴细胞培养液（LCF）中的增殖和活化情况与在10%胎牛血清（FBS）中培养的细胞进行比较。肿瘤浸润淋巴细胞（TIL）在LCF中呈指数级增殖长达46天，产生的细胞数量比在FBS中培养的细胞多近100000倍。由于细胞生长是这些技术中的一个限制因素，T细胞在LCF中的这种快速生长可能对过继性免疫疗法和基因疗法产生重要影响。
