Rajasekar R, Augustin A
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Am J Respir Cell Mol Biol. 1994 Jan;10(1):79-84. doi: 10.1165/ajrcmb.10.1.8292384.
The double negative (CD4-CD8-) alpha beta+ T cells constitute about 20% of all alpha beta+ T cells in murine lungs. We find that in BALB/c mice 60% of the double negative alpha beta+ pulmonary T cells express receptors of the V beta 8 family whereas only 33% of single positive (CD4+/CD8+) pulmonary T cells express V beta 8. However, in C57BL/6 mice, equal frequencies (25%) of double negative and single positive alpha beta+ pulmonary T cells express V beta 8. The high frequency of double negative V beta 8+ pulmonary T cells is dominantly inherited in (C57BL/6 X BALB/c) F1 offsprings. Further studies exclude the involvement of classic MHC region genes in determining the level of V beta 8 usage among double negative pulmonary T cells. Upon exposure to mycobacterial antigens, double negative alpha beta+ pulmonary T cells are co-enriched in vitro in parallel with gamma delta+ T cells.
双阴性（CD4-CD8-）αβ+ T细胞约占小鼠肺中所有αβ+ T细胞的20%。我们发现，在BALB/c小鼠中，60%的双阴性αβ+肺T细胞表达Vβ8家族受体，而单阳性（CD4+/CD8+）肺T细胞中只有33%表达Vβ8。然而，在C57BL/6小鼠中，双阴性和单阳性αβ+肺T细胞表达Vβ8的频率相等（25%）。双阴性Vβ8+肺T细胞的高频率在（C57BL/6×BALB/c）F1后代中呈显性遗传。进一步的研究排除了经典MHC区域基因在决定双阴性肺T细胞中Vβ8使用水平方面的作用。暴露于分枝杆菌抗原后，双阴性αβ+肺T细胞在体外与γδ+ T细胞同时被共富集。
