Lallemand A V, Ruocco S M, Gaillard D A
Laboratoire Pol Bouin, CHU Reims, Inserm U. 314, France.
Int J Dev Biol. 1993 Sep;37(3):491-5.
The expression and distribution of laminin and type IV collagen, two major components of the basement membrane, were investigated at the epithelio-mesenchymal interface of the human developing tracheal glands from 10 to 37 weeks of gestation. The localization of these molecules was assessed by indirect immunoperoxidase and indirect immunofluorescence staining and correlated to morphogenesis and epithelial cell differentiation. Laminin and type IV collagen were detected as early as 10 weeks of gestation in a continuous, linear pattern in the basement membrane surrounding the epithelial tracheal tube. By 12 weeks of gestation the basement membrane developed large openings at the tips of the budding glands beneath poorly differentiated cells, concomitant with the onset of morphogenetic movements. The remodeling of the basement membrane led to branching epithelial morphogenesis. The maturation and the functional differentiation of the secretory cells appeared later in the epithelium, when the basement membrane was strongly labeled with both anti-laminin and anti-type IV collagen antibodies, after 24 weeks of gestation. At this time the basement membrane became regular and thick and the maturation of serous cells increased progressively. These results suggest that the remodeling of the basement membrane takes place very early during gestation and that the morphogenesis and the maturation of the tracheal glands are rapidly achieved in humans.
在妊娠10至37周的人类发育中气管腺的上皮-间充质界面，研究了基底膜的两种主要成分层粘连蛋白和IV型胶原的表达及分布。通过间接免疫过氧化物酶和间接免疫荧光染色评估这些分子的定位，并将其与形态发生和上皮细胞分化相关联。早在妊娠10周时，在围绕气管上皮管的基底膜中就检测到层粘连蛋白和IV型胶原呈连续的线性模式。到妊娠12周时，基底膜在未分化细胞下方的萌芽腺尖端形成大的开口，同时形态发生运动开始。基底膜的重塑导致上皮形态发生分支。分泌细胞的成熟和功能分化在妊娠24周后上皮中出现得较晚，此时基底膜被抗层粘连蛋白和抗IV型胶原抗体强烈标记。此时基底膜变得规则且增厚，浆液细胞的成熟逐渐增加。这些结果表明，基底膜的重塑在妊娠早期就发生了，并且气管腺的形态发生和成熟在人类中迅速完成。
