小鼠血管紧张素II 2型受体的cDNA及基因组DNA的克隆

Cloning of the cDNA and the genomic DNA of the mouse angiotensin II type 2 receptor.

作者信息

Ichiki T, Herold C L, Kambayashi Y, Bardhan S, Inagami T

机构信息

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.

出版信息

Biochim Biophys Acta. 1994 Jan 19;1189(2):247-50. doi: 10.1016/0005-2736(94)90072-8.

DOI:10.1016/0005-2736(94)90072-8

PMID:8292631

Abstract

Of the two major isoforms of the angiotensin II receptors, type 1 (AT1) and type 2 (AT2), little is known about the structure and features of AT2. We cloned a mouse AT2 cDNA from a mouse fetus cDNA library and an AT2 genomic DNA from a 129SV mouse genomic DNA library. The amino acid sequence of the mouse AT2 (363 residues) deduced from a mouse cDNA clone showed seven membrane-spanning domains. Amino acid identity of the mouse AT2 with mouse AT1 is 37%, and 98% with rat AT2. The genomic DNA (4.4 kb) contained three exons and two introns and the entire coding region was contained in the third exon.

摘要

在血管紧张素II受体的两种主要亚型中，1型（AT1）和2型（AT2），人们对AT2的结构和特征了解甚少。我们从小鼠胎儿cDNA文库中克隆了小鼠AT2 cDNA，并从129SV小鼠基因组DNA文库中克隆了AT2基因组DNA。从小鼠cDNA克隆推导的小鼠AT2（363个氨基酸残基）的氨基酸序列显示有七个跨膜结构域。小鼠AT2与小鼠AT1的氨基酸同一性为37%，与大鼠AT2的氨基酸同一性为98%。基因组DNA（4.4 kb）包含三个外显子和两个内含子，整个编码区包含在第三个外显子中。