Tsuzuki S, Ichiki T, Nakakubo H, Kitami Y, Guo D F, Shirai H, Inagami T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Biochem Biophys Res Commun. 1994 May 16;200(3):1449-54. doi: 10.1006/bbrc.1994.1613.
The gene of human angiotensin II type 2 (AT2) receptor was isolated from a genomic DNA library prepared from human placenta. The coding region of the human AT2 receptor gene was contained in a single exon coding segment of the gene indicating an intronless structure of the coding region. The amino acid sequence of human AT2 receptor deduced from its nucleotide sequence has 363 amino acids and shows a high degree of sequence identity to rat and mouse receptor sequences. Specific binding of [125I]Sar1Ile8-angiotensin II was demonstrated in COS-7 cells transfected with a plasmid containing the human AT2 sequence. Scatchard analysis and ligand displacement profile were typical of the AT2 receptor. Reverse transcription-polymerase chain reaction analysis showed that AT2 receptor mRNA was expressed in adult uterus and pheochromocytoma.
人血管紧张素II 2型（AT2）受体基因是从人胎盘制备的基因组DNA文库中分离出来的。人AT2受体基因的编码区包含在该基因的单个外显子编码片段中，表明编码区无内含子结构。从其核苷酸序列推导的人AT2受体的氨基酸序列有363个氨基酸，与大鼠和小鼠受体序列具有高度的序列同一性。在用含有人AT2序列的质粒转染的COS-7细胞中证明了[125I]Sar1Ile8 -血管紧张素II的特异性结合。Scatchard分析和配体置换曲线是AT2受体的典型特征。逆转录-聚合酶链反应分析表明，AT2受体mRNA在成年子宫和嗜铬细胞瘤中表达。
