Shelud'ko N S, Kropacheva I V, Permiakova T V
Biokhimiia. 1993 Dec;58(12):1936-44.
Changes in the properties of natural actomyosin suspensions, such as turbidity, light scattering, volume concentration, particle size and particle size distribution during superprecipitation (SPP) of actomyosin in media of different compositions have been investigated. Two sets of changes indicating the existence of two SPP types--the "immediate" SPP characterized by a rapid one-step rise in turbidity and the "spontaneous" SPP, when the slow increase in turbidity follows the clear phase, have been revealed. In both cases the mechanism of the increase in the suspension turbidity is assumed to be similar and is conditioned by an increase in the refraction index (density) of the particles; however, the ways of formation of dense particles are different. During the "immediate" SPP, the particle density increases due to particle striction, whereas during the "spontaneous" SPP dense particles are formed as a result of ordered lateral association of myosin and actin filaments.
研究了在不同组成介质中,肌动球蛋白超沉淀(SPP)过程中天然肌动球蛋白悬浮液的性质变化,如浊度、光散射、体积浓度、粒径和粒径分布。揭示了两组变化,表明存在两种SPP类型——以浊度快速一步上升为特征的“即时”SPP,以及在清相之后浊度缓慢增加的“自发”SPP。在这两种情况下,悬浮液浊度增加的机制被认为是相似的,并且是由颗粒折射率(密度)的增加所决定的;然而,致密颗粒的形成方式是不同的。在“即时”SPP过程中,颗粒密度由于颗粒收缩而增加,而在“自发”SPP过程中,致密颗粒是肌球蛋白和肌动蛋白丝有序横向缔合的结果。