Sooranna S R, Moss J, Contractor S F
Academic Department of Obstetrics and Gynaecology, Chelsea and Westminster Hospital, London, UK.
Cell Tissue Res. 1993 Dec;274(3):619-25. doi: 10.1007/BF00314560.
Trophoblast cells were cultured on microporous membrane filters. After incubation at different times with gold-conjugated ligands, the cells were processed for electron microscopy. Gold particles indicating the presence of both IgG and LDL appeared in a time-dependent manner in coated pits and coated vesicles. LDL-gold appeared primarily within lysosomes whereas approximately 50% of the internalized IgG-gold appeared within vesicles (diameters ranging from 35 to 80 nm) near the basal regions of the cell. These vesicles may be the protective mechanism which prevents IgG breakdown during transcytosis across trophoblast cells, thus allowing transport of the intact molecule to the fetus.
滋养层细胞在微孔膜滤器上培养。在用金标记的配体孵育不同时间后，对细胞进行电子显微镜处理。表明存在IgG和低密度脂蛋白（LDL）的金颗粒以时间依赖性方式出现在被膜小窝和被膜小泡中。LDL-金主要出现在溶酶体内，而内化的IgG-金约50%出现在细胞基部区域附近的小泡（直径范围为35至80nm）内。这些小泡可能是一种保护机制，可防止IgG在跨滋养层细胞的转胞吞作用过程中分解，从而使完整分子能够转运至胎儿。
