Olivereau M, Olivereau J M, Yamashita K, Matsuda K, Kikuyama S
Laboratoire de Physiologie, Institut Océanographique, Paris, France.
Cell Tissue Res. 1993 Dec;274(3):627-30. doi: 10.1007/BF00314561.
An antiserum was prepared against the recently purified bullfrog (bf) growth hormone (GH); it was applied to sections of brain and pituitary of three urodele (Ambystoma, Pleurodeles and Cynops) and three anuran (Xenopus, Bufo vulgaris and B. japonicus) species. No immunostaining was obtained in the urodele pituitary, being consistent with the results of immunoblot analysis of the pituitary homogenate. In the three anuran species, strong immunoreactivity was observed in GH cells that were concentrated in the posterodorsal region of the pars distalis. No GH-like immunoreactivity was detectable in the brain of any of the species. A comparison using adjacent sections stained with anti-bf prolactin (PRL) confirmed the anteroventral localization of PRL cells. Colocalization of GH and PRL was not apparent. These data suggest that the molecular structure of amphibian GHs is considerably different between anurans and urodeles. The antiserum used in the present work shows a high species specificity, recognizing only anuran GHs. In contrast anti-bfPRL labeled PRL cells in all the amphibian species studied in the present work, suggesting that PRLs possess common amino acid sequences recognized by the anti-bfPRL.
制备了一种针对最近纯化的牛蛙（bf）生长激素（GH）的抗血清；将其应用于三种有尾目动物（蝾螈、肋突螈和东方蝾螈）以及三种无尾目动物（非洲爪蟾、普通蟾蜍和日本蟾蜍）的脑和垂体切片。在有尾目动物的垂体中未获得免疫染色，这与垂体匀浆的免疫印迹分析结果一致。在三种无尾目动物中，在远侧部后背部区域集中的GH细胞中观察到强免疫反应性。在任何一种动物的脑中均未检测到类似GH的免疫反应性。使用抗bf催乳素（PRL）染色的相邻切片进行比较，证实了PRL细胞的腹前侧定位。GH和PRL的共定位不明显。这些数据表明，无尾目动物和有尾目动物的两栖类GH分子结构有很大差异。本研究中使用的抗血清显示出高物种特异性，仅识别无尾目动物的GH。相比之下，抗bfPRL在本研究中所研究的所有两栖类物种中标记了PRL细胞，这表明PRL具有抗bfPRL识别的共同氨基酸序列。
