Campantico E, Guastalla A, Patriarca E
Gen Comp Endocrinol. 1985 Jan;57(1):110-6. doi: 10.1016/0016-6480(85)90206-0.
The indirect immunofluorescence procedure was used to localize prolactin (PRL)- and somatotrophin (STH)- producing cells in the pituitary distal lobe from Hyla arborea adult specimens. The following mammalian antisera were employed: rabbit anti-ovine PRL, antibovine PRL, anti-human PRL, anti-rat PRL, anti-ovine STH, anti-bovine STH, anti-human STH; monkey anti-rat STH. Immunocytochemical staining was suppressed by solid phase absorption of both anti-PRL and anti-STH with the specific antigen. Absorption of anti-PRL with STH and of anti-STH with PRL did not appreciably affect immunocytochemical staining. Treatment with the two antisera revealed two different reactive cell types, both acidophils. Using PRL antisera a strong fluorescence was found in the large acidophils located chiefly in the rostro-central and ventral areas of the distal lobe sagittal sections. A somewhat weaker fluorescence was observed using STH antisera in the fewer, small acidophils mostly concentrated in the dorso-caudal region and only sparsely scattered in the other areas of the pars distalis. Strikingly, the overall pattern of localization shown by the two cell types is similar to their already known distribution in the pituitary distal lobe of some other species of urodele and anuran amphibians.
采用间接免疫荧光法对成年无斑雨蛙垂体远侧叶中产生催乳素（PRL）和生长激素（STH）的细胞进行定位。使用了以下哺乳动物抗血清：兔抗羊PRL、抗牛PRL、抗人PRL、抗大鼠PRL、抗羊STH、抗牛STH、抗人STH；猴抗大鼠STH。抗PRL和抗STH与特异性抗原的固相吸收抑制了免疫细胞化学染色。用STH吸收抗PRL以及用PRL吸收抗STH对免疫细胞化学染色没有明显影响。用这两种抗血清处理后发现了两种不同的反应性细胞类型，均为嗜酸性细胞。使用PRL抗血清时，在主要位于远侧叶矢状切片 rostro - 中央和腹侧区域的大型嗜酸性细胞中发现了强烈荧光。使用STH抗血清时，在较少的小型嗜酸性细胞中观察到较弱的荧光，这些小型嗜酸性细胞大多集中在背尾区域，仅稀疏地散布在远侧部的其他区域。引人注目的是，这两种细胞类型显示的总体定位模式与它们在一些其他有尾目和无尾目两栖动物垂体远侧叶中已知的分布相似。
