Dancis A, Yuan D S, Haile D, Askwith C, Eide D, Moehle C, Kaplan J, Klausner R D
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20892.
Cell. 1994 Jan 28;76(2):393-402. doi: 10.1016/0092-8674(94)90345-x.
We report the identification and characterization of CTR1, a gene in the yeast S. cerevisiae that encodes a multispanning plasma membrane protein specifically required for high affinity copper transport into the cell. The predicted protein contains a methionine- and serine-rich domain that includes 11 examples of the sequence Met-X2-Met, a motif noted in proteins involved in bacterial copper metabolism. CTR1 mutants and deletion strains have profound deficiency in ferrous iron uptake, thus revealing a requirement for copper in mediating ferrous transport into the cell. Genetic evidence suggests that the target for this requirement is the FET3 gene (detailed in a companion study), predicted to encode a copper-containing protein that acts as a cytosolic ferro-oxidase. These findings provide an unexpected mechanistic link between the uptake of copper and iron.
我们报告了CTR1的鉴定和特性，CTR1是酿酒酵母中的一个基因，它编码一种多跨膜质膜蛋白，是细胞高亲和力铜转运所特需的。预测的蛋白质包含一个富含甲硫氨酸和丝氨酸的结构域，其中有11个Met-X2-Met序列实例，这是在参与细菌铜代谢的蛋白质中发现的一种基序。CTR1突变体和缺失菌株在亚铁摄取方面存在严重缺陷，从而揭示了铜在介导亚铁转运进入细胞中的必要性。遗传学证据表明，这种必要性的靶点是FET3基因（在一项配套研究中有详细描述），预计该基因编码一种含铜蛋白，作为胞质铁氧化酶发挥作用。这些发现为铜和铁的摄取之间提供了一个意想不到的机制联系。
