慢性产前和产后铅离子暴露会增加成年大鼠前脑的[3H]MK801结合位点。

Chronic prenatal and postnatal Pb2+ exposure increases [3H]MK801 binding sites in adult rat forebrain.

作者信息

Guilarte T R, Miceli R C, Altmann L, Weinsberg F, Winneke G, Wiegand H

机构信息

Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205.

出版信息

Eur J Pharmacol. 1993 Oct 1;248(3):273-5. doi: 10.1016/0926-6917(93)90054-t.

DOI:10.1016/0926-6917(93)90054-t

PMID:8293792

Abstract

We have measured the binding of [3H]MK801 to the N-methyl-D-aspartate (NMDA) receptor-ion channel in membrane preparations from adult rat forebrain exposed to lead (Pb2+) during gestation, lactation, and postweaning. Our results indicate a 30.9% increase in the number of [3H]MK801 binding sites in Pb2+ exposed animals relative to controls. No significant changes in the affinity constant were observed. The level of blood Pb2+ for which such changes were measured was 13.9 +/- 2.8 micrograms/dl. These results indicate that alterations in the NMDA receptor-ion channel complex are present at blood Pb2+ levels which are environmentally relevant and suggest that chronic Pb2+ exposure during development can influence the NMDA receptor complex in the adult rat brain.

摘要

我们已经测量了[3H]MK801与成年大鼠前脑膜制剂中N-甲基-D-天冬氨酸（NMDA）受体离子通道的结合情况，这些成年大鼠在妊娠、哺乳期和断奶后暴露于铅（Pb2+）环境中。我们的结果表明，与对照组相比，暴露于Pb2+的动物中[3H]MK801结合位点的数量增加了30.9%。未观察到亲和常数有显著变化。测量到这种变化时的血铅水平为13.9±2.8微克/分升。这些结果表明，在与环境相关的血铅水平下，NMDA受体离子通道复合物会发生改变，这表明发育过程中慢性暴露于Pb2+会影响成年大鼠大脑中的NMDA受体复合物。