Dong J, Iuchi S, Kwan H S, Lu Z, Lin E C
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Gene. 1993 Dec 22;136(1-2):227-30. doi: 10.1016/0378-1119(93)90469-j.
The cpxA gene of Escherichia coli K-12 encodes a membrane-associated sensor element of a two-component signal transduction system in bacteria. The cognate regulator element, however, has not yet been definitively identified. A 2.1-kb segment upstream from cpxA was amplified by polymerase chain reaction, cloned and sequenced. An open reading frame encoding 232 amino acids was found. It showed high homology to the regulator elements of two-component transduction systems. The newly identified gene, designated as cpxR, may encode the cognate protein receiving signals from CpxA.
大肠杆菌K-12的cpxA基因编码细菌中双组分信号转导系统的一个膜相关传感元件。然而，同源调节元件尚未得到明确鉴定。通过聚合酶链反应扩增、克隆并测序了cpxA上游2.1 kb的片段。发现了一个编码232个氨基酸的开放阅读框。它与双组分转导系统的调节元件具有高度同源性。新鉴定的基因命名为cpxR，可能编码从CpxA接收信号的同源蛋白。
